How to concentrate the virus in water by aluminum trichloride precipitation

In recent years, the outbreak of water-borne viral diseases has been reported in a large number in the world. A large number of studies have confirmed that external environmental water bodies and silt are the main places for virus survival cycle, and thus frequently cause epidemics of infectious diseases. However, in the various water bodies in the living environment, in addition to domestic sewage, the concentration of the virus contained in the water is often very low. With the existing detection methods, the purpose of detecting the virus directly from the water without concentration is still not achieved. At the same time, the dose of food-borne virus is very low, and 100 virions can cause infection. The virus has strong viability under in vitro conditions, and has strong resistance to various physical and chemical factors. It is resistant to ether and weak acid. It can not be inactivated by chloroform, repeated freezing and thawing, and ultrasonic treatment. Therefore, the presence of a small amount of virus in the water environment still poses a great threat to human health. As a direct raw material for purified water, aluminum trichloride has attracted widespread attention.

In China, although PCR-based rapid detection of viruses has been established, the pretreatment technology for water samples has not found a very ideal method for virus concentration, which cannot effectively remove inhibitors in samples. These factors are severely constrained. Detection and prevention of foodborne viruses in water. To grasp the basic state of virus contamination in various water bodies, the concentration of virus in the sample is the key to successful detection. In addition, in the standards implemented by most countries in the world, the detection standards of viruses and their indicator organisms are not clearly stated. The bottom line is that there is no effective method established so far. In view of this, in response to the hotspots and existing problems of virus research in water, the first issue of this issue in 2009 published Xiao Xiaoxia and Wu Qingping’s method for concentrating viruses in two different water bodies, tap water and sewage. On the basis of the existing detection and concentration methods, through continuous innovation, solve the main problems existing in the existing methods, explore the key points that restrict the method of virus concentration in water, and evaluate the advantages and disadvantages and practicability of different methods. The biggest advantage of the aluminum trichloride precipitation method is that it can be applied to most water samples with different water quality characteristics, in particular, it can overcome the defects that the membrane filtration method has strict limits on the concentration of suspended solids due to easy clogging. In addition, as far as the detection conditions are concerned, the method does not require a filtering device, etc., and the imported Yang electrical filter membrane is omitted, which is more convenient from the point of application promotion, and has low cost and cost. For systematic research and detection of common food-borne virus in water in China. Have a certain application value.